Felipe, Stela Mirla da Silva and Pacheco, Christina and Martins, Jonathan Elias Rodrigues and Freitas, Raquel Martins de and Oliveira, Paulo Elesson Guimarães de and Mendes, Sávio Victor Diógenes and Alves, Juliana Osório and Ceccatto, Vânia Marilande (2023) Optimization of RNA Extraction Protocol for Rat Skeletal Muscle Samples. Journal of Applied Life Sciences International, 26 (1). pp. 10-16. ISSN 2394-1103
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Abstract
Aims: The present study aimed to stablish and characterize an optimized protocol conformation to obtain adequate RNA quality from rodents skeletal muscle samples for sequencing studies.
Place and Duration of Study: The in vivo experiments and analyses were performed in the Laboratory of Biochemistry and Gene Expression – LABIEX of the Superior Institute of Biomedical Science – ISCB from the State University of Ceará - UECE. Between 2017-2020.
Methodology: Were used 23 samples from male Wistar rat skeletal muscle, specifically from soleus muscle. Total RNA extraction was performed using the classic TRIzol® method and commercial kit, merging steps from both. Capillary electrophoresis in the Bioanalyzer platform was used for RNA quality evaluation.
Results: (C) Analyzes of adapted protocol RNA concentration, RIN and rate 28S/18S showed satisfactory results. 28S/18S Ribosomal bands appear well defined, without small traces, which indicates RNA with high integrity and without contamination of genomic DNA.
Conclusion: Obtained RNA quality and integrity data satisfied the exigencies for posterior RNA-seq.
Item Type: | Article |
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Subjects: | Journal Eprints > Biological Science |
Depositing User: | Managing Editor |
Date Deposited: | 21 Jan 2023 06:37 |
Last Modified: | 02 Apr 2024 04:17 |
URI: | http://repository.journal4submission.com/id/eprint/1609 |