Shen, Yi and Cui, Jingjing and Zhang, Shuang and Wang, Yuqing and Wang, Jia and Su, Yuxin and Xu, Dongsheng and Liu, Yihan and Guo, Yating and Bai, Wanzhu (2022) Temporal alteration of microglia to microinfarcts in rat brain induced by the vascular occlusion with fluorescent microspheres. Frontiers in Cellular Neuroscience, 16. ISSN 1662-5102
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Abstract
Microglia, the resident immune cells in the central nervous system, can monitor the microenvironment and actively respond to ischemic stroke and other brain injuries. In this procedure, microglia and neurons can cross-talk via transmembrane chemokine, Fractalkine (CX3CL1), to impact one another. We used a rat model of multifocal microinfarcts induced by the injection of fluorescent microspheres into the right common carotid artery and examined the morphological alteration of blood vessels, microglia, astrocytes, and neurons at 6 h, 1, 7, and 14 days after modeling, along with neurobehavioral tests and the staining of CX3CL1 in this study. Our results demonstrated that in the infarcted regions, astrocytes and microglia activated in response to neuronal degeneration and upregulation of cleaved caspase-3, which occurred concurrently with vascular alteration and higher expression of CX3CL1. We provided sequential histological data to shed light on the morphological changes after modeling, which would help in the identification of new targets and the choice of the ideal time window for therapeutic intervention in ischemic stroke.
Item Type: | Article |
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Subjects: | Journal Eprints > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 29 Mar 2023 05:25 |
Last Modified: | 14 Mar 2024 04:31 |
URI: | http://repository.journal4submission.com/id/eprint/1686 |