Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry

C., G. Kouadio-Yapo and L., Hasseine and P., Delaunay and P., Marty and M., Gari-Toussaint (2018) Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry. African Journal of Microbiology Research, 12 (4). pp. 68-72. ISSN 1996-0808

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Abstract

This study aimed to identify yeasts directly from positive blood cultures by MALDI-TOF mass spectrometry. This was a 5-month prospective study (February to June, 2015) carried out on bottles of blood cultures from in-patients at the Hôpital de l’ARCHET at the CHU, Nice. Positive blood culture broth was analysed by MALDI-TOF mass spectrometry using Sodium Dodecyl Sulphate (SDS) as the lysis buffer. Out of 64 samples, 51 (79.6%) were identified by MALDI-TOF mass spectrometry at the species level and 12 strains (18.7%) at the genus level. The isolated yeasts fell into six species: Candida albicans 37.2%, Candida glabrata 31.4%, Candida parapsilosis 15.7%, Candida tropicalis 5.9%, Candida krusei 5.9%, and Candida lusitaniae 3.9%. In comparison, 95.3% of species were identified from cultured colonies, the main ones being C. albicans (37.1%), C. glabrata (30.7%) and C. parapsilosis (16.1%). Identification of yeasts from blood culture bottles by MALDI-TOF mass spectrometry is a fast, reliable technique. However, analysis of colonies remains the best technique for identification and for antifungal imaging in order to refer the patient to the most appropriate therapy.

Item Type: Article
Subjects: Journal Eprints > Biological Science
Depositing User: Managing Editor
Date Deposited: 30 Mar 2023 06:24
Last Modified: 16 Mar 2024 04:44
URI: http://repository.journal4submission.com/id/eprint/1694

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