Genetic Diversity of Mutant Lines of Rice Using RAPD Markers

Present study was conducted to characterized mutant lines of rice variety Rajendra Mahsuri-1. Genetic diversity in rice mutant lines (M3) was characterised by using RAPD markers among them at the molecular level. Altogether 78 alleles were amplified, of which 53 were polymorphic. Polymorphic information content (PIC) ranged from 0.082 for OPA2 to 0.424 for OPA3. Among the primers used, OPA3, OPA7, OPA8, OPA13 and OPB6 had a remarkably higher number of variants with greater gene diversity and rice mutant lines discrimination ability. Major allele frequency was found higher in case of OPA2, OPA4, OPA9 and OPB7. Using a similarity coefficient in the numerical taxonomic approach of classification, the mutant lines were differentiated and classified into different groups. Principal coordinate analysis based two-dimensional plotting of genetic profiles completely supported the results obtained from the hierarchical classification of mutant lines were distinctly discriminated from the remaining lines.