Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR

van Zogchel, Lieke M. J. and Zappeij-Kannegieter, Lily and Javadi, Ahmad and Lugtigheid, Marjolein and Gelineau, Nina U. and Lak, Nathalie S. M. and Zwijnenburg, Danny A. and Koster, Jan and Stutterheim, Janine and van der Schoot, C. Ellen and Tytgat, Godelieve A. M. (2021) Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR. Cancers, 13 (1). p. 150. ISSN 2072-6694

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Abstract

mRNA RT-qPCR is shown to be a very sensitive technique to detect minimal residual disease (MRD) in patients with neuroblastoma. Multiple mRNA markers are known to detect heterogeneous neuroblastoma cells in bone marrow (BM) or blood from patients. However, the limited volumes of BM and blood available can hamper the detection of multiple markers. To make optimal use of these samples, we developed a multiplex RT-qPCR for the detection of MRD in neuroblastoma. GUSB and PHOX2B were tested as single markers. The adrenergic markers TH, GAP43, CHRNA3 and DBH and mesenchymal markers POSTN, PRRX1 and FMO3 were tested in multiplex. Using control blood and BM, we established new thresholds for positivity. Comparison of multiplex and singleplex RT-qPCR results from 21 blood and 24 BM samples from neuroblastoma patients demonstrated a comparable sensitivity. With this multiplex RT-qPCR, we are able to test seven different neuroblastoma mRNA markers, which overcomes tumor heterogeneity and improves sensitivity of MRD detection, even in those samples of low RNA quantity. With resources and time being saved, reduction in sample volume and consumables can assist in the introduction of MRD by RT-qPCR into clinical practice.

Item Type: Article
Subjects: Journal Eprints > Medical Science
Depositing User: Managing Editor
Date Deposited: 07 Jan 2023 07:41
Last Modified: 16 Sep 2023 05:28
URI: http://repository.journal4submission.com/id/eprint/221

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