Larvicidal Efficacy of the Combination of Citrus aurantiifolia (Rutaceae) and Lippia chevalieri (Verbenaceae) Methanolic Extracts against Anopheles gambiae Giles (Diptera Culicidae), Malaria Vector

Ignareki, Damas Dainone and Dadji, Gisele Aurelie Foko and Younoussa, Lame and Baudelaire, Elie Njantou and Dicko, Amadou and Rup-Jacques, Sandrine and Djapoum, Carine Bertille Theno and Nkondjio, Antonio and Tamesse, Joseph Lebel and Fomena, Abraham (2023) Larvicidal Efficacy of the Combination of Citrus aurantiifolia (Rutaceae) and Lippia chevalieri (Verbenaceae) Methanolic Extracts against Anopheles gambiae Giles (Diptera Culicidae), Malaria Vector. International Journal of TROPICAL DISEASE & Health, 44 (13). pp. 9-20. ISSN 2278-1005

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Abstract

The growing resistance of mosquito vectors towards synthetic insecticides constitutes a major challenge in the malaria control and synergistic action of two or more extracts may decrease the risk of insect resistance. For that, the methanolic extracts of Citrus aurantiifolia peels and Lippia chevalieri leaves were tested individually and in combination of Anopheles gambiae larvae at concentrations of 7.5, 15, 22.5 and 30 mg/mL in the laboratory according to the standard protocol of WHO. Extraction yields were 6.72 and 4.62 for C. aurantiifolia and L. chevalieri respectively. Saponins, terpenoids, flavonoids, tannins and polyphenols were present in the extracts of C. aurantiifolia and L. chevalieri. The 2 plants extracts tested individually and combined, caused a significant (P < .001) larvicidal concentration-dependent activity 24 hours’ post-exposure. C. aurantiifolia peels was revealed as the most potent against the mosquito larvae (LC50 = 9.82 mg/mL), while only the binary combination 50%Ca + 50%Lc (CI: 155.22; SF: 1.55) induced a synergistic action against Anopheles gambiae larvae. Thus, the combination 50%Ca + 50%Lc might be recommended as a natural bio-insecticide in mosquito control program to prevent malaria.

Item Type: Article
Subjects: Journal Eprints > Medical Science
Depositing User: Managing Editor
Date Deposited: 12 Sep 2023 11:58
Last Modified: 12 Sep 2023 11:58
URI: http://repository.journal4submission.com/id/eprint/2517

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