Optimization of Somatic Embryogenesis in Ferula assa-foetida L. (a Rare Medicinal Plant)

Otroshy, Mahmoud and Roozbeh, Shirin (2015) Optimization of Somatic Embryogenesis in Ferula assa-foetida L. (a Rare Medicinal Plant). Annual Research & Review in Biology, 5 (5). pp. 439-448. ISSN 2347565X

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Abstract

Experiments was carried out to examine the effect of abscisic acid (ABA), source of carbon and temperature on secondary somatic embryogenesis and somatic embryo germination of Ferula assa-foetida L. Cotyledonary somatic embryos were cultured on MS (Murashig and Skoog, 1962) medium supplemented with 0, 1.0, 1.5 or 2.0 mg/l ABA and on MS medium with 3% mannitol, sorbitol or sources. The highest number of secondary somatic embryos (35.94) was observed on MS medium devoid of ABA at 25°C, but the addition of abscisic acid, sorbitol or manitol to the MS basal medium significantly reduced the formation of secondary somatic embryos at 5°C. The concentration of abscisic acid had a significant effect on the rate of secondary somatic embryogenesis. Among the carbon source studied, mannitol significantly suppressed the induction of secondary somatic embryos. Abscisic acid had a positive effect on the percentage of somatic embryo germination. Higher germination was observed with1.0 or 1.5 mg/l ABA, but it was not significantly affected by sorbitol or manitol. Abscisic acid increased germination at 15°C and 25°C while sorbitol and manitol enhanced it at 5°C.

Item Type: Article
Subjects: Journal Eprints > Biological Science
Depositing User: Managing Editor
Date Deposited: 14 Oct 2023 04:26
Last Modified: 14 Oct 2023 04:26
URI: http://repository.journal4submission.com/id/eprint/2583

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