Facile In vitro Glass Slide Light Microscopy Method Using Tetracycline to Visualise a Repetitive Pattern in Aerial Plant Root Tips Filamentous Network: An Advanced Study

The major goal of this paper is to present a simple light microscopy technique for visualising plant root filaments. In vitro tests on freshly plucked human hair roots revealed the antibiotic Tetracycline's (TE) harmful effect on soft tissue, which was severe enough to allow detection of an underlying filamentous skeleton in a previous paper. In this paper, TE was tested in a similar way to in vitro tests, but this time with aerial plant roots immersed in liquid Tetracycline. Plant aerial root tissue cells appeared to interact with Tetracycline, allowing for the revealing of an underlying filamentous network, as shown in images and video recordings. Tetracycline biosorption was seen in these filaments, indicating that they may have a keratin foundation. It should be noted that a literature search revealed similar, albeit visually distinct, presentations of root filaments produced via scanning electron microscopy. The method described here could be used in conjunction with existing root function research methodologies. The essential minimal material and equipment is restricted to a light microscope, glass slides, chosen biological material, water, and powder Tetracycline, which is one of the most significant advantages. Second, the speed with which data are obtained would provide researchers with a preliminary or possibly final correct conclusion.