Impact of Alcohol Exposure on the Composition of HeLa-Derived Extracellular Vesicles

Jones, Leandra B. and Kumar, Sanjay and Curry, Aliyah J. and Price, Jayde S. and Krendelchtchikov, Alexandre and Crenshaw, Brennetta J. and Bell, Courtnee’ R. and Williams, Sparkle D. and Tolliver, Tambre A. and Saldanha, Sabita N. and Sims, Brian and Matthews, Qiana L. (2021) Impact of Alcohol Exposure on the Composition of HeLa-Derived Extracellular Vesicles. In: Recent Progress in Microbiology and Biotechnology Vol. 5. B P International, pp. 152-170. ISBN 978-93-90768-59-2

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Abstract

Extracellular vesicles are nanosized vesicles that are under intense investigation for their role in intercellular communication. Extracellular vesicles have begun to be examined for their role in disease protection and their role as disease biomarkers and/or vaccine agents. With the high mortality rate of cervical cancers, more information is needed regarding the detection and treatment of the diseased. In this regard, several investigators have begun to examine extracellular vesicles and their role in disease protection. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of extracellular vesicles derived from the cervical cancer line, HeLa. The HeLa cells were cultured in exosome-free media and were either mock-treated (control) or treated with 50 mM or 100 mM of alcohol for 24 h and 48 h. Our results demonstrated that alcohol significantly impacts HeLa cell viability and exosome biogenesis/composition. Importantly, our studies demonstrate the critical role of alcohol on HeLa cells, as well as HeLa-derived extracellular vesicle biogenesis and composition. Specifically, these results indicate that alcohol alters extracellular vesicles’ packaging of heat shock proteins and apoptotic proteins. Extracellular vesicles serve as communicators for HeLa cells, as well as biomarkers for the initiation and progression of the disease. HeLa cells used the primary model in this study, the research findings may have substantial implications on diagnostics and therapy for a variety of cell types and organ systems. Further investigation is needed to elucidate the mechanism(s) involved in these processes.

Item Type: Book Section
Subjects: Journal Eprints > Medical Science
Depositing User: Managing Editor
Date Deposited: 31 Oct 2023 09:50
Last Modified: 31 Oct 2023 09:50
URI: http://repository.journal4submission.com/id/eprint/3011

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