FOUR DIFFERENT STAINING METHODS FOR DETECTION OF MITOTIC FIGURES IN ORAL SQUAMOUS CELL CARCINOMA

In the diagnosis and prognosis of oral squamous cell carcinoma (OSCC) and oral epithelial dysplasia, evaluation of mitotic figures (MFs) is often performed. Numerous techniques, like microscopy, flow cytometry, nucleotide radio labelling, as well as immuno-histochemistry, have been developed throughout time to evaluate the MFs. The most modern techniques are more precise for everyday usage, but because of time and money constraints, they are less practical. In the current research, mitotic figures in various stages of oral squamous cell carcinoma were examined using Haematoxylin and Eosin stain, Crystal Violet stain, Giemsa stain and Toluidine Blue stain. Based on statistical comparison, the number of mitotic figures seen in each of the four distinct stains was compared.