Chemical and Antimicrobial Analyses of Juniperus chinensis and Juniperus seravschanica Essential Oils and Comparison with Their Methanolic Crude Extracts

Khamis, Al-Dhafri S. and Chai, Lay Ching and Venuti, Valentina (2021) Chemical and Antimicrobial Analyses of Juniperus chinensis and Juniperus seravschanica Essential Oils and Comparison with Their Methanolic Crude Extracts. International Journal of Analytical Chemistry, 2021. pp. 1-8. ISSN 1687-8760

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Abstract

Juniperus chinensis and Juniperus seravschanica are commonly used in the traditional folk medicine to treat microbial infection. In this study, the essential oils obtained from the leaves of J. chinensis growing in Malaysia and J. seravschanica growing in Oman were analysed by head space-solid phase microextraction-gas chromatography mass spectrometry (HS-SPME-GC-MS) and screened for antimicrobial activities against Escherichia coli (NCTC 10418), Pseudomonas aeruginosa (NCTC 10662), Bacillus subtilis ATCC6059, Micrococcus luteus (ATCC 9341), Staphylococcus aureus (NCTC 6571), and methicillin-resistant S. aureus (MRSA; ATCC 33591). To compare the antimicrobial activities of extracts using different extraction methods, methanol extraction was performed to obtain crude extracts from the leaves of J. chinensis and J. seravschanica for antimicrobial analysis. *e HS-SPME-GS-MS analysis of the essential oils from the leaves of J. chinensis and J. seravschanica identified 37 and 36 components, respectively. Essential oils from these two species had distinctive chemical component profiles, with α-pinene (27.2%) as the major component of J. chinensis essential oil, while dl-limonene (45.2%) constitutes the major component of J. seravschanica essential oil. Essential oils of these two species shared only six similar terpenoids compounds: α-pinene, β-pinene, c-elemene, sabinene, elemol, and 3-cyclohexen-1-ol. Overall, the essential oils showed antimicrobial activities against all the six bacterial strains tested, with the highest antagonistic activity against M. luteus and B. cereus; while, methanolic crude extracts showed the highest activities against S. aureus and MRSA strains. *e methanolic crude extracts demonstrated significantly higher antibacterial activity against the Gram-positive bacteria (p < 0.005); while, the essential oils of Juniperus did not show significant differences between Gram-positive and Gram-negative bacteria. Future studies are needed to investigate the active compounds present in the essential oils and methanolic crude extracts that confer the selectivity in the antimicrobial activity

Item Type: Article
Subjects: Journal Eprints > Chemical Science
Depositing User: Managing Editor
Date Deposited: 16 Dec 2022 12:45
Last Modified: 19 Sep 2023 07:23
URI: http://repository.journal4submission.com/id/eprint/307

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