Calcite Type Fired Shell Powder of Bivalve Corbicula japonica Improved Mal-Functions of Liver in Animal and in Calcite-Ingested Clients

It has been inherited for a long preriod in a small village that the fired shell powder of bivalve
Corbicula japonica has been working against liver disorder without any scientific evidences.
Experiment was initiated by the village office requirement to ascertain an anti-hepatitis activity of the
fired shell powder of the Corbicula japonica. Shell of the Corbicula japonica was fired to analyze a
crystal structure by the X-ray diffraction apparatus, followed by animal tests by hepatitis model Long
Evans Cinnamon (LEC) rat. In C57BL/6 mouse model additional biofunctions like immune activation,
lipid controlling, anti-alcoholic damage activity and liver cells proliferation tests were evaluated. The
fired shell powder of the Corbicula japonica (calcite type) fired at 500°C for 2 hrs demonstrated the
liver function improving activity in LEC rat model via orally administerationt. The calcite type shell
powder fed-LEC rats elongated survival times by the improving acute hepatitis symptoms, however,
the Corbicula japonica aragonite and Ruditapes philippinarum calcite did not work effectively. Further
Corbicula japonica calcite lowered AST(glutamic oxaloacetic transaminase) ALT (glutamic pyruvic
transaminase), TBiI (total bilirubin) and IAP (immunosuppressive acidic protein) value in serum, and
enforced cellular immune by increasing NK (natural killer) cells activity. Cytokines generation as TNF-
α (tumor necrosis factor), IL-2 (interleukin) and IFN-γ (interferon) in spleen cells cultivation assay was
not clearly admitted. In the alcohol-fed mouse test, the Corbicula japonica calcite reduced alcoholic
caused damages in liver and lowered lipid level in serum.