In vitro ANTIFUNGAL EVALUATION OF PREPARED CARBENDAZIM NANOEMULSION FOR EFFECTIVE TRANSLOCATION IN FUNGAL CELL

This study was made to explore the tendency of effective permeability of antifungal carbendazim by formulating as o/w nanoemulsion (NE). The NE was made by optimizing the concentrations of oleic acid as oil, Tween 20 as surfactant and acetone as co-surfactant, by unprompted emulsification procedure. They were characterized for transparency, drug content, compatibility, pH, viscosity, stability and size by TEM (transmission electron microscopic) analysis. In vitro antifungal assay were conducted against two phytopathogens, Alternaria alternata and Dreschlera oryzae. The transparent Nano emulsion with narrow size range of oil droplets, appropriate pH and viscosity was obtained. TEM analysis indicated the development of distinct nanosized droplets with size ranging between 80-140 nm. The NEs were stable under variable temperature conditions and have improved permeation through fungal spore cells leading to better results in comparison to the marketed Wettable Powder or suspension of Carbendazim.