OPTIMIZATION OF EXTRACTION OF DNA FROM Brassica oleracea var. botrytis (Cauliflower) USING RESPONSE SURFACE METHODOLOGY

In this study, response surface methodology (RSM) was undertaken to optimize and model the DNA extraction from Brassica oleracea var. botrytis (Cauliflower). The design variables were the volume of the lysis buffer; the volume of chloroform and the ratio between the volume of NaCl / ethanol (v/v) and the volume of the aqueous phase recovered after purification while response variables were DNA concentration and purity.

The results of this study estimated the effects of the three parameters on the specified responses, as well as the regression coefficients of the developed linear mathematical models. On the other hand, the experimental values of responses agreed with those predicted by the two models employed. The optimal values ​​of the three parameters studied were also determined.