Analysis of the Anti-inflammatory and Antioxidant Potential of the Fractions Obtained From the Ethyl Acetate Partition of the Leaves of Diplopterys Pubipetala

Almeida, Clarice Avelar and Franca, Flavia Dayrell and Chaves, Miriam Martins and Santos, Kamylla Teixiera and Santos, Maria Clara Ferreira and Menezes, Elytania Veiga and Araujo, Sandra de Sousa and Araujo, Cleiber Lucan Alves and Royo, Vanessa de Andrade (2022) Analysis of the Anti-inflammatory and Antioxidant Potential of the Fractions Obtained From the Ethyl Acetate Partition of the Leaves of Diplopterys Pubipetala. European Journal of Medicinal Plants, 33 (5). pp. 1-11. ISSN 2231-0894

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Abstract

Aims: The objective of this work was to analyze the anti-inflammatory, cytotoxic and antioxidant activity of the fractions of ethyl acetate from the leaves of D. pubipetala.

Methodology: Liquid-liquid partitioning was performed, the ethyl acetate partition was eluted in classical liquid chromatography, followed by thin layer chromatography. Antioxidant activity was performed by the DPPH photochlorimetric method and the levels of interleukin (IL‑6), tumor necrosis factor α (TNF‑α), interleukin-10 (IL-10) and nitric oxide (NO) were identified in supernatants of cell cultures.

Results: Fractions F4, F5 and F10 showed antioxidant activity. The F10 fraction indicated efficient anti-inflammatory activity by the production of IL-10 and nitric oxides. Fractions F4, F5 and F9 stimulated the production of IL-6 and TNF-α.

Conclusion: These results indicate a possible synergistic action of the extract and the partition of ethyl acetate, since after fractionation the potential presented in previous studies was lower.

Item Type: Article
Subjects: Journal Eprints > Medical Science
Depositing User: Managing Editor
Date Deposited: 03 Jan 2023 07:31
Last Modified: 13 Mar 2024 04:21
URI: http://repository.journal4submission.com/id/eprint/606

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