Shan, Lu and Dai, Zongjie and Wang, Qinhong (2021) Advances and Opportunities of CRISPR/Cas Technology in Bioengineering Non-conventional Yeasts. Frontiers in Bioengineering and Biotechnology, 9. ISSN 2296-4185
pubmed-zip/versions/1/package-entries/fbioe-09-765396/fbioe-09-765396.pdf - Updated Version
Download (1MB)
Abstract
Non-conventional yeasts have attracted a growing interest on account of their excellent characteristics. In recent years, the emerging of CRISPR/Cas technology has improved the efficiency and accuracy of genome editing. Utilizing the advantages of CRISPR/Cas in bioengineering of non-conventional yeasts, quite a few advancements have been made. Due to the diversity in their genetic background, the ways for building a functional CRISPR/Cas system of various species non-conventional yeasts were also species-specific. Herein, we have summarized the different strategies for optimizing CRISPR/Cas systems in different non-conventional yeasts and their biotechnological applications in the construction of cell factories. In addition, we have proposed some potential directions for broadening and improving the application of CRISPR/Cas technology in non-conventional yeasts.
Item Type: | Article |
---|---|
Subjects: | Journal Eprints > Biological Science |
Depositing User: | Managing Editor |
Date Deposited: | 20 Feb 2023 06:29 |
Last Modified: | 26 Feb 2024 04:19 |
URI: | http://repository.journal4submission.com/id/eprint/754 |