Platelet-Derived Growth Factor-A Overexpression Correlates with Atrial Fibrosis in the Patients with Atrial Fibrillation Secondary to Rheumatic Valvular Disease

Su, Mengxia and Zhao, Rui and Wang, Xu and Yang, Yulu and Ma, Feng and Pan, Junqiang (2022) Platelet-Derived Growth Factor-A Overexpression Correlates with Atrial Fibrosis in the Patients with Atrial Fibrillation Secondary to Rheumatic Valvular Disease. International Journal of Clinical Medicine, 13 (11). pp. 501-514. ISSN 2158-284X

[thumbnail of ijcm_2022112316141927.pdf] Text
ijcm_2022112316141927.pdf - Published Version

Download (1MB)

Abstract

Objective: To investigate the relationship between platelet-derived growth factor-A (PDGF-A) and atrial fibrosis in patients who have developed atrial fibrillation (AF) secondary to rheumatic valvular disease. Methods: 84 selected patients participated in the current study who have developed rheumatic heart disease and were going to have a cardiac surgical operation. In the current study, whole subjects were divided into two group, they were atrial fibrillation (AF) group (the quantity is thirty-nine) and sinus rhythm (SR) group (the quantity is forty-five). Before the operation, complete clinical data was available for the whole patients. During the operation, the right atrial tissue (0.3 - 0.5 mm3) was disserted from every patient. Right atrial fibrosis was observed by Masson staining and the distribution of PDGF-A in right atrium specimen was observed by immunohistochemistry. RT-PCR techniques were applied to admeasure the mRNA expressions of PDGF-A in patients’ atrial tissue. At the same time, western-Blot techniques were employed to admeasure the protein expressions of PDGF-A. Results: In baseline clinical characteristics, in both AF group and SR group, there was no apparently difference between them (P > 0.05); compared with SR group, the diameters of left atrium and right atrium in AF group were apparently increased (P < 0.05). The results of Masson staining revealed that the atrial tissue fibrosis was clearer in AF group, and collagen volume fraction in the AF group was evidently exceeding SR group (P < 0.05). The expressions of PDGF-A’s mRNA and protein from right atrial tissue in the AF group were evidently greater than SR group (P < 0.05). The mRNA and protein expressions of PDGF-A and the right atrium diameter go hand in hand. Conclusion: Atrial remodeling plays an important role in patients with valvular atrial fibrillation; PDGF-A in patients with AF was highly expressed in the right atrial, and was closely related with atrial fibrosis.

Item Type: Article
Subjects: Journal Eprints > Medical Science
Depositing User: Managing Editor
Date Deposited: 31 Jan 2023 06:19
Last Modified: 20 Mar 2024 04:20
URI: http://repository.journal4submission.com/id/eprint/808

Actions (login required)

View Item
View Item